Plate 8

Images of Litoria fallax produced from cryopreserved testicular macerates (Gosner Stages 20–46). (A) Freshly hatched, free-swimming tadpole; 5 mm, Gosner Stage 20. (B) Tadpole nearing metamorphosis, hind legs beginning to develop; Gosner Stage 35, 20–30 mm. (C) Tadpole with fully developed hind legs; Gosner Stage 40, 30–40 mm. (D) Metamorph, eruption of the forelimb and resorption of the tail commenced; Gosner Stage 42, 30–40 mm. (E) Juvenile frog; tail resorbed; Gosner Stage 46, 10–20 mm. (F) Sexually mature male, 20–30 mm. Reproduced with permission from Upton R, Clulow S, Mahony MJ, Clulow J (2018). Generation of a sexually mature individual of the Eastern dwarf tree frog, Litoria fallax, from cryopreserved testicular macerates: proof of capacity of cryopreserved sperm derived offspring to complete development. Conservation Physiology 6, coy043.